[ { "id": 491215, "publisher": 695644, "username": "Lysine", "avatar": "https://img-avatar.eduzhixin.com/avatar/259014f7590078b629e755bb23738de3/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003e非常非常非常不好意思\u003cimg src=\"https://static.eduzhixin.com/json/forum/image/emoji/images/拉普拉斯3.png\" width=\"44\" height=\"44\" alt=\"拉普拉斯3.png\" data-emoji=\"true\" data-non-image=\"true\"\u003e\u003c/p\u003e\u003cp\u003e不过这个东西肯定不能叫逆转录吗嘛毕竟转录是核酸之间的概念，而且蛋白质到DNA是连跨两级传递，怎么叫得有高人来想，佬是怎么看的？\u003c/p\u003e", "thumb_up_num": 6, "comment_num": 7, "created_at": "1777379466", "has_thumb_up": 0, "comment_list": [ { "id": 762478, "publisher": 695644, "username": "Lysine", "avatar": "https://img-avatar.eduzhixin.com/avatar/259014f7590078b629e755bb23738de3/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003e感觉这个寡聚氨基酸序列到特定三联重复DNA序列就有那么一丝当初从poly(U), poly (A) RNA序列体外翻译出寡聚氨基酸的意思，感觉可以用这种方法加上分子技术研究蛋白质到DNA的互作关系…………\u003c/p\u003e", "thumb_up_num": 3, "did": 66724, "created_at": "1777379775", "rid": 491215, "reply_uid": 0, "reply_uname": "", "has_thumb_up": 0, "avatar_dress_up_url": "", "avatar_dress_up_name": "", "avatar_dress_up_id": 0, "user_code": "UID_868e79456654bee4b1e5cd064fa34081" }, { "id": 762595, "publisher": 400291, "username": "Questioner", "avatar": "https://img-avatar.eduzhixin.com/avatar/21762a7d771524db7d15234ccecf18e1/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003e只是互作的话就不算太稀奇了吧，最早的基因编辑就有蛋白和特定碱基序列的配对了\u003c/p\u003e", "thumb_up_num": 2, "did": 66724, "created_at": "1777392071", "rid": 491215, "reply_uid": 695644, "reply_uname": "Lysine", "has_thumb_up": 0, "avatar_dress_up_url": "https://img-forum.eduzhixin.com/forum/zhixin/dress_up/avatar/yinwuzhechuji.png", "avatar_dress_up_name": "引物者-初级", "avatar_dress_up_id": 9, "user_code": "UID_19a4a624da24087fd2ff43c278091fd8" }, { "id": 762651, "publisher": 695644, "username": "Lysine", "avatar": "https://img-avatar.eduzhixin.com/avatar/259014f7590078b629e755bb23738de3/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003e确实ZFN和TALEN是蛋白配对核酸\u003cimg src=\"https://static.eduzhixin.com/json/forum/image/emoji/images/赞1.png\" width=\"44\" height=\"44\" alt=\"赞1.png\" data-emoji=\"true\" data-non-image=\"true\"\u003e大佬能不能畅想一下它的应用\u003c/p\u003e", "thumb_up_num": 1, "did": 66724, "created_at": "1777437937", "rid": 491215, "reply_uid": 400291, "reply_uname": "Questioner", "has_thumb_up": 0, "avatar_dress_up_url": "", "avatar_dress_up_name": "", "avatar_dress_up_id": 0, "user_code": "UID_868e79456654bee4b1e5cd064fa34081" }, { "id": 762696, "publisher": 400291, "username": "Questioner", "avatar": "https://img-avatar.eduzhixin.com/avatar/21762a7d771524db7d15234ccecf18e1/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003e应用当然是——\u003c/p\u003e\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\u003cp\u003e基因编辑啦hhh\u003c/p\u003e", "thumb_up_num": 2, "did": 66724, "created_at": "1777466056", "rid": 491215, "reply_uid": 695644, "reply_uname": "Lysine", "has_thumb_up": 0, "avatar_dress_up_url": "https://img-forum.eduzhixin.com/forum/zhixin/dress_up/avatar/yinwuzhechuji.png", "avatar_dress_up_name": "引物者-初级", "avatar_dress_up_id": 9, "user_code": "UID_19a4a624da24087fd2ff43c278091fd8" }, { "id": 762715, "publisher": 695644, "username": "Lysine", "avatar": "https://img-avatar.eduzhixin.com/avatar/259014f7590078b629e755bb23738de3/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003e如果说这个新玩意可以解决脱靶的问题或者比Cas13, Cpf2a之类的更优化那肯定会红得发紫，如果不能的话Cas系统还是目前最厉害的它只配当辅助技术了hhhhhh\u003c/p\u003e", "thumb_up_num": 1, "did": 66724, "created_at": "1777469637", "rid": 491215, "reply_uid": 400291, "reply_uname": "Questioner", "has_thumb_up": 0, "avatar_dress_up_url": "", "avatar_dress_up_name": "", "avatar_dress_up_id": 0, "user_code": "UID_868e79456654bee4b1e5cd064fa34081" }, { "id": 762718, "publisher": 695644, "username": "Lysine", "avatar": "https://img-avatar.eduzhixin.com/avatar/259014f7590078b629e755bb23738de3/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003e另外不知道佬有没有看过杨Sir在我爱生化公众号上发的自己关于这个东西的看法(他甚至因此做了个梦关于这个)，还是蛮精彩的，另外好久没看到发这种关于前沿帖子的了突然想念Chicken佬(泪目，新生代论坛生物学术谁来做😥😥😥)\u003c/p\u003e", "thumb_up_num": 1, "did": 66724, "created_at": "1777469970", "rid": 491215, "reply_uid": 400291, "reply_uname": "Questioner", "has_thumb_up": 0, "avatar_dress_up_url": "", "avatar_dress_up_name": "", "avatar_dress_up_id": 0, "user_code": "UID_868e79456654bee4b1e5cd064fa34081" }, { "id": 762750, "publisher": 400291, "username": "Questioner", "avatar": "https://img-avatar.eduzhixin.com/avatar/21762a7d771524db7d15234ccecf18e1/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003ehh，之前那么多厉害的大佬用这些也是做基因编辑最出色不是（），所以在这之前最好的应用可能真的只有基因编辑了。而且大概也是由于自然界中这几个蛋白本来就是用来基因编辑？所以说这个用来做基因编辑完全是开玩笑啦，毕竟术业有专攻。同理它如果用来“沉默”特定的DNA/RNA 片段感觉肯定也不如专门刚这个的RNAi或者类似的蛋白优秀了，所以最重大的意义肯定是逆转录了\u003c/p\u003e\u003cp\u003e我专门去看了一下杨sir的公众号，如果说这样弄出DNA更便宜的话肯定是很重大的突破了！\u003c/p\u003e\u003cp\u003e至于再想有什么别的用的话，就像CRISPER可以魔改cas蛋白一样。蛋白碱基互作是找到特定序列的东西，具体对它再做什么还可以再魔改（但是人为设计太难，最好就是像现在这样先有自然界的模板再在这个基础上做些什么）\u003c/p\u003e\u003cp\u003e不过其实我有点难以想象对游离核苷酸能魔改什么，cas可以魔改成只敲掉一边。逆转录能魔改啥呢？难道合成的时候顺便加修饰？我不知道\u003c/p\u003e", "thumb_up_num": 2, "did": 66724, "created_at": "1777473974", "rid": 491215, "reply_uid": 695644, "reply_uname": "Lysine", "has_thumb_up": 0, "avatar_dress_up_url": "https://img-forum.eduzhixin.com/forum/zhixin/dress_up/avatar/yinwuzhechuji.png", "avatar_dress_up_name": "引物者-初级", "avatar_dress_up_id": 9, "user_code": "UID_19a4a624da24087fd2ff43c278091fd8" } ], "avatar_dress_up_url": "", "avatar_dress_up_name": "", "avatar_dress_up_id": 0, "user_code": "UID_868e79456654bee4b1e5cd064fa34081" }, { "id": 496446, "publisher": 429576, "username": "一坨蚊子鱼", "avatar": "https://img-avatar.eduzhixin.com/avatar/dbe15fe2d3c4628351777d8130a32aeb/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003eemmm\u003c/p\u003e\u003cp\u003e之前看别的视频科普\u003c/p\u003e\u003cp\u003e我觉得叫“拓印”之类的比较好\u003c/p\u003e\u003cp\u003e就是通过蛋白质构成模板压出来一串固定的DNA链\u003c/p\u003e", "thumb_up_num": 3, "comment_num": 0, "created_at": "1779298507", "has_thumb_up": 0, "comment_list": [ ], "avatar_dress_up_url": "", "avatar_dress_up_name": "", "avatar_dress_up_id": 0, "user_code": "UID_8dec70831e865007360d8646e9572ed0" }, { "id": 500527, "publisher": 679404, "username": "UE_^Upsilon暝黳", "avatar": "https://img-avatar.eduzhixin.com/avatar/4997ae3ed2d09900d38710773a8a4711/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003e题外话：\u003c/p\u003e\u003cp\u003e听说关于阿尔茨海默症的淀粉样蛋白病变假说被证是伪论文并退稿了？！\u003c/p\u003e\u003cp\u003e岂不是一大堆的这方向的研究白研究了\u003c/p\u003e", "thumb_up_num": 4, "comment_num": 0, "created_at": "1781080649", "has_thumb_up": 0, "comment_list": [ ], "avatar_dress_up_url": "", "avatar_dress_up_name": "", "avatar_dress_up_id": 0, "user_code": "UID_2579aa0e4a22f3cdaca4f5aba379adb1" }, { "id": 504538, "publisher": 449816, "username": "F.Herleta(苏德美次炸了", "avatar": "https://img-avatar.eduzhixin.com/avatar/b07e79c07ebfcb610bfb3b4601cb3620/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "这个是特异性的而且复制出来的只有一小段特定的DNA那些自媒体在乱说。不具有普遍性。", "thumb_up_num": 1, "comment_num": 1, "created_at": "1783005643", "has_thumb_up": 0, "comment_list": [ { "id": 778644, "publisher": 449816, "username": "F.Herleta(苏德美次炸了", "avatar": "https://img-avatar.eduzhixin.com/avatar/b07e79c07ebfcb610bfb3b4601cb3620/s.jpg?x-oss-process=style/avatar\u0026t=202609122211", "content": "\u003cp\u003e赞同作者观点，个人认为逆转录这个说法并不严谨。因为像艾滋这种能够传递遗传信息的才叫逆转录吧。而这个DNA序列只是重复的一小段。我估计教材应该会在中心法则或者选必3里提一嘴\u003c/p\u003e", "thumb_up_num": 0, "did": 66724, "created_at": "1783005884", "rid": 504538, "reply_uid": 0, "reply_uname": "", "has_thumb_up": 0, "avatar_dress_up_url": "", "avatar_dress_up_name": "", "avatar_dress_up_id": 0, "user_code": "UID_b4b47f1d6f286be513d4d15b6c3f6221" } ], "avatar_dress_up_url": "", "avatar_dress_up_name": "", "avatar_dress_up_id": 0, "user_code": "UID_b4b47f1d6f286be513d4d15b6c3f6221" } ]